DNA pHspAI2 (4118 bp) is a modified plasmid pHspAI1. Besides a gene of DNA-methyltransferase M.HspAI (recognition sequence 5'-GCGC-3') it contains a unique GlaI canonical site.
pFsp4HI1 (7634 bp) was obtained by a ligation of the vector pUC19/EcoRI and a fragment of genomic DNA of bacterial strain Flavobacterium species 4H, 4942 bp in length, that contained the gene of DNA-methyltransferase M.Fsp4HI
pHspAI1 (4118 bp) was obtained by a ligation of the vector pUC19/EcoRI and a fragment of genomic DNA of bacterial strain Haemophilus species A1, 1438 bp in length, that contained the gene of DNA-methyltransferase M.HspAI.