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| | | | A new methyl-directed site-specific endonuclease KroI recognizes and cuts DNA sequence 5-G^C(5mC)GGC-3 | | |
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| V.A. Chernukhin*, E.V. Kileva, J.E. Tomilova, A.A. Boltengagen, V.S. Dedkov, N.A. Mikhnenkova, D.A. Gonchar, L.N. Golikova, S.Kh. Degtyarev
* - corresponding author | | |
| | | | | | | | | | | We have discovered and purified a new methyl-directed site-specific DNA endonuclease KroI from bacterial strain Kocuria rosea 307. KroI recognizes and cuts DNA sequence 5-G↑C(5mC)GGC-3/3-CGG(5mC)C↓G-5 and doesnt cleave unmethylated DNA. A new enzyme cleaves both strands of the recognition sequence if one or two central cytosines are methylated. KroI is a first methyl-directed site-specific DNA endonuclease that recognizes the non-degenerate hexanucleotide sequence. Due to its ability to cleave only modified DNA KroI may find a practical application in genetic engineering experiments as well as in determination of DNA methylation status. | |
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This paper in Russian. 