| |  | | | | | | The Second Methyltransferase of the BstF5I Restriction–Modification System Is Homologous to the C-Terminal Domains of FokI and StsI Methylases | |
| | The bstF5IM-2 gene for the second DNA methyltransferase of Bacillus stearothermophilus F5 (M.BstF5I-2) was cloned and sequenced. On the chromosome, the gene is located downstream of the gene for M.BstF5I-1 and is oriented similarly. The protein encoded has conserved regions specific for adenine methylases of subclass D12. M.BstF5I-2 is homologous to the C-terminal domains of M.FokI and M.StsI and recognizes the same sites. M.BstF5I-1 modifies the DNA upper strand and M.BstF5I-2 modifies the other strand in the recognition site. | |
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| | | | | Multiplicity of Site-specific DNA Methyltransferases of the BstF5l Restriction-Modification System | |
| | A fragment located downstream of the genes for DNA methyltransferases of Bacillus stearothermophilus F5 (M.BstF5I-1 and M.BstF5I-2) was sequenced. The fragment contains a gene for another methylase. M.BstF5I-3. structurally and functionally similar to the N-terminal domain of M.FokI. Thus, in contrast to other restriction-modification systems, the BstF5I system includes three methylases, two being homologous to the individual M.FokI domains. | |
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