Murat A. Abdurashitov, Valery A. Chernukhin, Danila A. Gonchar, Vladimir S. Dedkov, Natalia A. Mikhnenkova, and Sergey Kh. Degtyarev
Research Journal of Pharmaceutical, Biological and Chemical Sciences, Volume 7, Issue 1, 2016 (January - February)
Methyl-directed site-specific DNA endonuclease GlaI recognizes and cleaves methylated DNA sequence RCGY. GlaI activity in hydrolysis of recognition sequence containing two 5-methylcytosines is noticeably lower than in the case of the site with four or three 5-methylcytosines. We have shown that addition of DMSO to the reaction mixture significantly increases GlaI activity in hydrolysis of sites with two 5- methylcytosines but doesn’t change a substrate specificity of the enzyme. A reaction buffer with DMSO may be used in epigenetic studies for GlaI digestion of eukaryotic genomes containing methylated CG pairs.