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The resctriction endonucleases detection in colonies of microorganisms Streptomyces and Nocardia

This email address is being protected from spambots. You need JavaScript enabled to view it. , Degtyarev S. Kh

Translated from APPLIED BIOCHEMISTRY and MICROBIOLOGY (Russia) Vol 28, 1992, #. 2, 309- 313

 

A simple technique is proposed for detection of restriction endonucleases in bacteria Streptomyces and Nocardia. The analysis was performed directly in the cells cultivated on Petri dishes and collected from colonies by an inoculation loop. The cells were treated with lysozyme, EDTA and Triton X-100. The lysates were tested for restriction endonucleases. The technique enables todetect the enzymes Nco I, Not I, Nru I I, Sfr 3031, and Sfi I in the lysates of corresponding strains-producers.

Read more: The resctriction endonucleases detection in colonies of microorganisms Streptomyces and Nocardia

A simple technique for detection of restriction endonucleases in bacterial colonies

Belavin P.A., This email address is being protected from spambots. You need JavaScript enabled to view it. , Degtyarev S. Kh.

Translated from APPLIED BIOCHEMISTRY AND MICROBIOLOGY (Russia), 1988, 24, N 1, 121 - 124

 

A simple technique is proposed for detection of bacterial restriction endonucleases. Analysis is performed directly in the cells from colonies cultivated on Petri dishes. The cells are collected by a microbiological loop into microtubes and then are treated with lysozyme and Triton X-100. After centrifugation of bacterial lysat the supernatant is tested for a presence of site-specific endonuclease activity. The technique enables to analyze about 100 colonies for 3-4 hours.

Read more: A simple technique for detection of restriction endonucleases in bacterial colonies