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Nucleotide sequence of plasmid DNA pHspAI2

DNA pHspAI2 (4118 bp) is a modified plasmid pHspAI1. Besides a gene of DNA-methyltransferase M.HspAI (recognition sequence 5'-GCGC-3') it contains a unique GlaI canonical site.



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TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CTCTTTCCAA TTTTCAATCT
ATCTGCAATA TCTTTTTCGT TTTTAAAACC ATCTTTAGCT GTTTTAGATC CTAATTCTAC
CAGATTCATT TTATTTACCT TTATTTATAG AGATTAGTAA TTTTTCTGCG ATTTTCTGTA
TTACATTAAC CGTCATCGCA TTTCCAGCTT GGGATAAAAT CTTACTATCG GGTAATTTAT
AATCAATGGC TTTTTGAGCA ATCTCTTTCG GAAATCCTTG TAATAATAAA GCCTCGTAAC
CACTTAACTT ATATAATTGA TTATCCTTGA CATATAAAAT CCCGTGCCTA CCTGTTCTAA
GCGTTGGTAT TTTATTTTCC ATAGAATCCG TAAAATCTGA CTGTCTCCAA TCTATTACCT
GATAATTTTT CTCATATATT TGATCTAAAC TTATTCTATC CTTATTGTAT TTATTATTAA
TATAATTTTC AAAAGTTTTA TTTATCAAAA ATTTATATTT ACCATCCCCA ATTAAATAAT
TTGACAACTC ATTTTCATTA TTGCTTTTAG GCTCAGGAAA GACATAATCA GAATGATGTA
TATCTTTTCT TATTCCAACA AAATAGATCC GTTCTCTCAT TTGTGGCGTA CCATAAAATT
GACTATCTAA TACTTTATAA TACACATCAT AGTTACAACT TTCTAATATT TCTAAGATTG
ATTTTAAAGT TTCACCTTGG TTATGATTTA CTAATCCCTT AACATTTTCT AAAATAAAAA
ATGGTACTTT TTTCACTTTC AAAATATTGC TTAAATAATA TATTATTTGC CCTCTATTAT
CTAGAAAACC ATCTCTTTTC CCAATGATAG AAAATGTTTG GCAAGGAAAT CCTGCAATCA
TTAAATCAAA ATCAGGTAAA GATTTTGGAG CAAGTTGAGT TAAATCACCT AAATTACGAC
TATCATTAAA AAATATTTCA TATGTTTTTG CAGGATTAAG ATCGACTTCG CTATGAGCAA
TACATTCCAT CCCGTTAAAC TCTAAGCCTA ATCGCCCCCC ACCAATACCT GCACAAAAAT
CAATAAACCT TAGTTTTTCC ATTAAAAATC TCCTGAACTA AAAAATAGTT TTATTTTGCA
TACCTATAAT TTTTCTGAAT TACCCAAGGC TGATGTTCAA TAATAATCTC ATCTTACTTT
TGAAAAGTTA CTACCCTTAT CTTTTATTTT TCTCTCAAAG AACTATTTAA TAAAATTTTA
TTCGTGTAGT TTGCATAAAA TGAAATCTCT TTACTCACTA ATTTTAAACC CTAACAATAA
AATCTCATCA ATAACAATTT ATACCACTCA ACTAATTCCT CAGCAATTTG CGTAATTCTC
TTATCATAAA AATGAAAAAT AACTCCAGGC ATTTCACCCG AGTTATCATA AATTAACGCT
TCATCACAAG ATAAAAGGGC TGTTATTGAA TTCGAGCTCG GTAGCGCGCG CTCCTCTAGA
GTCGACCTGC AGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA
TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA
GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC